BAD Knockout cell line (NCI-H1975)

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > KO Cell Lines > BAD Knockout cell line (NCI-H1975)

     gRNA Plasmid Bank

     Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

     Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

     Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

     Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

BAD Knockout cell line (NCI-H1975) Order

BAD Knockout cell line (NCI-H1975)
Catalog#
YKO-H1099
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BAD Knockout cell line (NCI-H1975)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BAD Knockout cell line (NCI-H1975)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Gene Name
BAD
Gene ID
572
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript BAD-201)
Validation: PCR+Sanger Sequencing
BAD Knockout cell line (NCI-H1975) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1975		 STR Info (Cell bank)
Cell Line:NCI-H1975[H-1975, H1975]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10131013
D7S820 88811
D16S539 912912
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BAD gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1975 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BAD gene knockout BAD Knockout cell line (NCI-H1975) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit
      inquiry   More details

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

Related Popular Products

Wild-type Cell Lines

  • NCI-H1975 cell line

    Human Lung Adenocarcinoma Cell Line(NCI-H1975)

    Price (USD): -
    Catalog#: YC-C106
    Size: 1*10^6
    Instruction: Human Lung Adenocarcinoma Cell Line(NCI-H1975)
      inquiry   More details

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:
      inquiry   More details

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us